| Applications | WB, IHC |
|---|---|
| Species Cross-Reactivity | H, R, M, Mk, Dg, C, Hm, Rb, Sh, Insect, Yeast |
| Molecular Weight | 55KD |
| Isotype | IgG1 |
| Recommended dilutions | WB:1:20,000-640,000 IHC: 1:1,000-2,000 Optimal dilutions should be determined by the end user |
| Specificity | The β-tubulin mouse monoclonal antibody can detect endogenous β-tubulin proteins. |
| Background | Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues. |
| Storage Buffer & Condition | PBS, pH 7.4, containing 0.02% kathon as Preservative and 50%Glycerol Store at -20°C. Do not aliquot the antibody. |
说明书.pdf
