| Applications | WB,IHC,IF,IP,ELISA |
|---|---|
| Species Cross-Reactivity | Human,Mouse,Rat |
| Molecular Weight | Observed:104kDa Calculated:104kDa |
| Source | Rabbit |
| Isotype | IgG,Kappa |
| Recommended dilutions | WB :1:500-1:2000 IHC: 1:200-1:1000 IF: 1:200-1:1000 IP: 1:200-1:1000 ELISA: 1:5000-1:20000. Optimal dilutions should be determined by the end user |
| Purification | Protein A |
| Specificity | Endogenous |
| Alternative Names | ACTN2 ;Alpha-actinin-2 ;Alpha-actinin skeletal muscle isoform 2 ; F-actin cross-linking protein |
| Gene Id | |
| Swiss Prot No | |
| RRID | AB_3740108 |
| Subcellular Location | Cytoplasm, myofibril, sarcomere, Z line . Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. |
| Expression | Expressed in both skeletal and cardiac muscle. |
| Background | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. |
| Storage Buffer & Condition | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA Store at -20°C. Stable for 12 months from date of receipt |
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