GAPDH Rabbit Polyclonal Antibody
Catalog:HX1832 Reactivity:H,M,R,Rb,Ch,Mk,Sh,Xenopus Applications:WB, IHC, IF Storage:Store at -20°C
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Applications WB, IHC, IF
Species Cross-Reactivity H,M,R,Rb,Ch,Mk,Sh,Xenopus
Molecular Weight 37KD
Source Rabbit
Isotype IgG
Recommended dilutions

WB: 1:5,000  IHC: 1:200

Optimal dilutions should be determined by the end user

Specificity Antibody can detects endogenous GAPDH protein.
RRID

AB_3101964

Background

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Storage Buffer & Condition PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50%Glycerol. Store at -20°C. Do not aliquot the antibody.
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