| Applications | WB,IHC-p,IF,ELISA |
|---|---|
| Species Cross-Reactivity | Human,Mouse,Rat |
| Molecular Weight | 52kDa |
| Source | Rabbit |
| Isotype | IgG |
| Recommended dilutions | WB :1:500-1:2000 IHC-p: 1:100-1:300 IF: 1:200 ELISA: 1:10000. Optimal dilutions should be determined by the end user |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Smad2 around the phosphorylation site of Thr220. AA range:186-235 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity | Phospho-Smad2 (T220) Polyclonal Antibody detects endogenous levels of Smad2 protein only when phosphorylated at T220. |
| Alternative Names | MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2; |
| Storage Buffer & Condition | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Store at -20°C. Stable for 12 months from date of receipt |
说明书.pdf
