WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human IRF3 around the phosphorylation site of Ser385. AA range:351-400
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
Phospho-IRF3 (S385) Polyclonal Antibody detects endogenous levels of IRF3 protein only when phosphorylated at S385.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):GAsSL