| Applications | WB,IHC-p,IF,ELISA |
|---|---|
| Species Cross-Reactivity | Human,Mouse |
| Molecular Weight | 70kD (Observed) |
| Source | Rabbit |
| Isotype | IgG |
| Recommended dilutions | WB 1:500-1:2000; IHC-p 1:100-1:300; ELISA 1:20000; IF 1:50-200 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AFX around the phosphorylation site of Ser197. AA range:164-213 |
| Specificity | Phospho-FoxO4 (S197) Polyclonal Antibody detects endogenous levels of FoxO4 protein only when phosphorylated at S197. |
| Alternative Names | FOXO4; AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1 |
| Gene Id | |
| Swiss Prot No | |
| Subcellular Location | Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm. |
| Expression | Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas. |
| Storage Buffer & Condition | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Store at -20°C. Stable for 12 months from date of receipt |
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