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Phospho-Chk2(T68) Polyclonal Antibody
Catalog：HX17242 Reactivity：Human,Mouse,Rat Applications：WB,IHC-p,IF,ELISA Storage：Store at -20°C
会员价：
50μl 100μl
Applications WB,IHC-p,IF,ELISA
Species Cross-Reactivity Human,Mouse,Rat
Molecular Weight 61kDa
Source Rabbit
Isotype IgG
Recommended dilutions

WB: 1:500-1:2000  IHC-p: 1:100-1:300 IF: 1:50-1:200  ELISA: 1:20000.Optimal dilutions should be determined by the end user
Immunogen The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Thr68. AA range:35-84
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity Phospho-Chk2 (T68) Polyclonal Antibody detects endogenous levels of Chk2 protein only when phosphorylated at T68.
Alternative Names CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2
Storage Buffer & Condition Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Store at -20°C. Stable for 12 months from date of receipt
Western Blot analysis of various cells using Phospho-Chk2 (T68) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit
Western blot analysis of lysates from Jurkat cells treated with UV, using Phospho-Chk2 (T68) Polyclonal Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Chk2 (Phospho-Thr68) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Chk2 (phospho Thr68) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Chk2 (phospho Thr68) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Chk2 (phospho Thr68) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-heart tissue. 1,Chk2 (phospho Thr68) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. 1,Chk2 (phospho Thr68) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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