| Applications | WB,IHC-p,IF,ELISA |
|---|---|
| Species Cross-Reactivity | Human,Mouse,Rat |
| Molecular Weight | 54kDa |
| Source | Rabbit |
| Isotype | IgG |
| Recommended dilutions | WB :1:500-1:2000 IHC-p: 1:100-1:300 IF: 1:200 ELISA: 1:5000. Optimal dilutions should be determined by the end user |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser317. AA range:286-335 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity | Phospho-Chk1 (S317) Polyclonal Antibody detects endogenous levels of Chk1 protein only when phosphorylated at S317. |
| Alternative Names | CHEK1; CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1 |
| Gene Id | |
| Swiss Prot No | |
| Background | The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. |
| Storage Buffer & Condition | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Store at -20°C. Stable for 12 months from date of receipt |
说明书.pdf
