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COL2A1 Polyclonal Antibody
Catalog：HX13543 Reactivity：Human,Mouse,Rat Applications：WB,IHC-p,IF,ELISA Storage：Store at -20°C
会员价：
50μl 100μl
Applications WB,IHC-p,IF,ELISA
Species Cross-Reactivity Human,Mouse,Rat
Molecular Weight 140kDa
Source Rabbit
Isotype IgG
Recommended dilutions

WB :1:500-1:2000  IHC-p: 1:100-1:300 IF: 1:200-1:1000  ELISA: 1:20000.

Optimal dilutions should be determined by the end user
Immunogen The antiserum was produced against synthesized peptide derived from human Collagen II. AA range:101-150
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity COL2A1 Polyclonal Antibody detects endogenous levels of COL2A1 protein.
Alternative Names COL2A1; Collagen alpha-1(II) chain; Alpha-1 type II collagen
Gene Id

1280
Swiss Prot No

P02458
Storage Buffer & Condition Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Store at -20°C. Stable for 12 months from date of receipt
Western Blot analysis of COLO205 cells using COL2A1 Polyclonal Antibody diluted at 1：1000
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,COL2A1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,COL2A1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,COL2A1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen II Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of COS7 cells, using Collagen II Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of rat-lung tissue. 1,COL2A1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. 1,COL2A1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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