首页 活动专区 新闻中心 关于我们 联系我们
Cleaved-Caspase-1 (D210) Polyclonal Antibody
Catalog：HX13390 Reactivity：Human,Mouse,Rat Applications：WB,IHC-p,IF,ELISA Storage：Store at -20°C
会员价：
50μl 100μl
Applications WB,IHC-p,IF,ELISA
Species Cross-Reactivity Human,Mouse,Rat
Molecular Weight 25kDa
Source Rabbit
Isotype IgG
Recommended dilutions

 WB :1:500-1:2000  IHC-p: 1:50-1:300 IF: 1:50-1:300    ELISA: 1:10000.Optimal dilutions should be determined by the end user
Immunogen The antiserum was produced against synthesized peptide derived from human Caspase-1. AA range:161-210
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity Cleaved-Caspase-1 (D210) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-1 protein resulting from cleavage adjacent to D210.
Alternative Names CASP1; IL1BC; IL1BCE; Caspase-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme.
Storage Buffer & Condition Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Store at -20°C. Stable for 12 months from date of receipt
Western Blot analysis of NIH-3T3 cells using Cleaved-Caspase-1 (D210) Polyclonal Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
首页 0购物车
加入购物车 立即购买