Phospho-eNOS (Ser1177) Polyclonal Antibody
Catalog:HX0952 Reactivity:Human,Mouse,Rat Applications:WB,IHC-p,IF,ELISA Storage:Store at -20°C
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Applications WB,IHC-p,IF,ELISA
Species Cross-Reactivity Human,Mouse,Rat
Molecular Weight 130-140kDa
Source Rabbit
Isotype IgG
Recommended dilutions

WB :1:500-1:2000  IHC-p: 1:50-1:200 IF:1:200  ELISA: 1:10000. Optimal dilutions should be determined by the end user

Immunogen The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Ser1176. AA range:1144-1193
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity Phospho-NOS3 (S1177) Polyclonal Antibody detects endogenous levels of NOS3 protein only when phosphorylated at S1177.
Alternative Names NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
Storage Buffer & Condition Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Store at -20°C. Stable for 12 months from date of receipt
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